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Eppendorf Á¦Ç° > Spectro photometer
Electroporation and Electrofusion:Multiporator

ºÐ·ù : Spectro photometer
ÆǸŰ¡°Ý :
¹è¼Ûºñ :   3500
¼ö·® :  

Ư¡

º» Multiporator´Â eukaryotic cellÀ» ´ë»óÀ¸·Î transient ¶Ç´Â stable transformationÀ» À§ÇÑ electroporation¿ë ±â±âÀ̸ç bacterai¿Í yeastÀÇ electroporationµµ °¡´ÉÇÔ.

Multiporator ¿ë Ư¼ö ¹öÆÛ¿Í Á¶ÇÕÀ» ÀÌ·ç¾î eukaryotic cellÀÇ electroporation ÀÇ È¿À²À» ³ôÀÏ ¼ö ÀÖ´Â ÃÖÀûÀÇ ½Ã½ºÅÛ Electroporation system ; adapted modularly to human or animal cells, plants.

  • ƯÇã Ãâ¿øÁßÀÎ electronic circuit ±â¼úÀº »ùÇà ÀúÇ׿¡ »ó°ü¾øÀÌ, ¼±ÅÃµÈ pulse parameter°¡ Á¤È®ÇÏ°Ô Àû¿ëµÉ ¼ö ÀÖµµ·Ï ÇÏ¿© ½ÇÇèÀÇ ÀçÇö¼ºÀ» ½ÇÇö½ÃÅ´.
  • Å¥ºª Å microsecond pulse¿Í ³·Àº field strength·Î ¼¼Æ÷ÀÇ »ýÁ¸À²ÀÌ ³ôÀ½.
  • Hypo-osmolar buffer´Â eukaryotic cellÀÇ transfection È¿À²¼ºÀ» ³ô¿©ÁÜ.
  • Hypo-osmolarÀÇ Æ¯Â¡ : (1)Å¥ºª ³» current flow¸¦ ³·Ã߾ ¼¼Æ÷ »óÇظ¦ ÀÏÀ¸Å³ ¼ö ÀÖ´Â pH gradientÀÇ Çü¼ºÀ» ¹æÁöÇÏ°í µ¶¼ºÀÌ ÀÖ´Â aluminium ionÀÇ À¯¸®¸¦ ³·Ãã. (2)Àü¾ÐÀ» ³·Ã߾ transfection rateÀ» ³ôÀÓ. (3) ¼¼Æ÷°¡ ¹°À» Èí¼öÇÏ¿© º¸´Ù µÕ±Ù ÇüŸ¦ À¯ÁöÇÏ¿© ¼¼Æ÷ Á÷°æÀ» ´ÃÀÓ
  • Compact, innovative and flexible system
  • Eukaryotic cellÀÇ electroporationÀÌ °¡´ÉÇÔ.

     Applications

  • Electroporation for the transient or stable transfection of eukaryotic cells
  • Electroporation of bacteria, yeasts, and other microorganisms
  • Electrofusion of mammalian cells, plant cells and oocytes


    Technical Information

    Pulse voltage: 20-1.200 V
    Pulse form: Exponentially diminishing, electronically controlled
    Time constant: 15-500 µs, in increments 5 µs
    Multiple pulsing: 1-99, with 1 min. time interval
    Interface: RS232


    Ordering Information

    Á¦Ç°¸í                                                                                          

    Multiporator¢ç

       Á¦Ç°¹øÈ£
    for eukaryotic cells 4308 000.015
    for eukaryotic cells, bacteria and yeasts 4308 000.023

    for eukaryotic cells and cell fusion, with 1 Helix fusion chamber

    and 1 Micro fusion chamber

    4308 000.031

    for eukaryotic cells, bacteria, yeasts and cell fusion, with

    1 Helix fusion chamber and 1 Micro fusion chamber

    4308 000.040
    Electroporation Buffer System
    Hypoosmolar, sterile, 100 ml 4308 070.501
    Isoosmolar, sterile, 100 ml 4308 070.510
    Electrofusion Buffer System
    Hypoosmolar, sterile, 100 ml 4308 070.528
    Isoosmolar, sterile, 100 ml 4308 070.536
    Helix fusion chamber, for cell fusion 4308 014.008
    Micro fusion chamber
    gap width 0.2 mm 4308 030.003
    gap width 0.5 mm 4308 031.000
    Stand for 10 Helix fusion chambers 4308 017.007

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